ISO INTERNATIONAL STANDARD 20179 First edition 2005-10-01 Water quality Determination of microcystins Method using solid phase extraction (SPE) and high performance liquid chromatography (HPLC) with ultraviolet (UV) detection Qualite de I'eau-Dosagedes microcystines-Methodeutilisant I'extractionenphase solide (SPE)et la chromatographie en phase liquideahauteperformance (CLHP)avec détectiondans I'ultraviolet (UV) Reference number ISO 20179:2005(E) @ISO2005 Copyright Intemational Organization for Standardization ISO20179:2005(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. ISO2005 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56·CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2005-All rights reserved Copyright Intemational Organization for Standardization ISO20179:2005(E) Contents Page Foreword IV Introductior 1 Scope 2 Normativereferences 3 Abbreviatedterms 4 Principle. 5 Reagents 6 Apparatus.. 7 Procedure. 8 Method performance characteristics and data..... 9 Test report. 10 Annex A(informative)Mass spectrometry (Ms)as an alternative detection. Annex B (informative) Typical chromatogram and absorption spectra . Annex C (informative) Precisiondata. Bibliography ii Copyright Intematinal organizaion frstandardzalon ghts reserved ISO 20179:2005(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO member bodies). The work of preparing International Standards is normally carried out through ISO International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent ISO20179waspreparedbyTechnical CommitteeISO/TC147,Waterquality,SubcommitteeSC2,Physical, chemicalandbiochemicalmethods @ISO 2005-All rights reserved Copyright Intemational Organization for Standardizatior ISO20179:2005(E) Introduction The user should be aware that particular problems could require the specification of additional conditions. Copyright Intemational organizaon frstandardzalonghts reserved CopyrightintematinalOrganizationforStandardzation INTERNATIONALSTANDARD ISO20179:2005(E) Water quality Determination of microcystins Method using solid phase extraction (SPE) and high performance liquid chromatography (HPLC) with ultraviolet (UV) detection WARNING- The method requires use of microcystin-containing solutions. Microcystins are highly hepatotoxictohumans.Laboratorywastesofmicrocystinsshall becollectedseparatelyanddisposed (NaClO) solution is also possible. Persons using this International Standard should be familiar with normal laboratory practice. This standarddoesnotpurporttoaddressallofthesafetyproblems,ifany,associate